THE ELECfROCHEMISTRY OF PROTEINS AND RELATED SUBSTANCES PART III. BOVINE SERUM ALBUMIN
نویسنده
چکیده
The electroreduction of bovine serum albumin (BSA) in pH 7.4 solution was studied at the hanging mercury drop electrode by double potential step chronocoulometry and cyclic voltammetry. and at a mercury pool electrode by controlled potential coulometry. The proposed mechanism involves reduction of an adsorbed monolayer of BSA (maximum coverage 3.3 1lC-2); at short times 3 or 4 disulfide bonds are reduced, the product remains adsorbed and may be reoxidized. On the coulometric time scale (hours) 8 or 9 of the total 17 disulfide bonds are broken and an insoluble product which cannot be oxidized is formed.
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